Scientist

About Glyphic:

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.

What we are looking for in you

We are seeking a Senior or Staff Scientist, Bioconjugation to join our Chemistry team at a pivotal stage in assay development. Reporting to the Senior Director of Chemistry, you will be a hands-on expert who designs, develops, and executes bioconjugation strategies for peptide and oligonucleotide components that are central to our single-molecule proteome sequencing assay.

The ideal candidate brings deep expertise in protein and peptide bioconjugation, site-specific chemical modification of amino acid sidechains, and oligonucleotide-biomolecule conjugation, with a track record of working in complex, multi-component systems (e.g., ADCs, therapeutic bioconjugates, or new bioconjugated components for assays). A successful candidate will have an innovative method-development mindset and will effectively build new approaches to making bioconjugates. This individual will thrive in a cross-functional environment where chemical solutions must be tightly integrated into assay design.

This role offers the unique opportunity to develop first-in-class conjugation chemistry that enables single-molecule protein sequencing. 

This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.

What you’ll do

Bioconjugation strategies:

• Partner with sample prep and assay development to screen and select conjugation chemistries for peptides and oligonucleotides that enable single molecule protein sequencing
• Design bioconjugation workflows using NHS-ester chemistry, maleimides, isothiocyanate chemistry, and click chemistry (CuAAC, SPAAC, IEDDA) 
• Prepare bioconjugation reagents to enable  studies from proof-of-concept through prototype-kitting.

Peptide chemistry:

• Develop labeling strategies based on Edman Chemistry
• Develop and apply selective C-terminal and N-terminal conjugation chemistries
• Develop site-specific labeling strategies for amino acid sidechains (Cys, Lys, Arg, Tyr, Trp, His, Asp/Glu) on native peptides. Screen protecting groups to enhance amino acid signal in the protein sequencing assay